Here you can find a list of labs working in the field of Synthetic Biology within Germany. In case your lab is not listed, feel free to get in touch with us and we will also provide your information here!
Dr. Arren Bar-Even
Systems and Synthetic Metabolism Lab, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1,14476 Potsdam-Golm , Germany
Our group aims to rewire central metabolism to provide highly optimized biotechnological solutions for humanity’s grand challenges and in doing so study the biochemical logic of cellular metabolism. We use a theoretical-computational approach to design novel synthetic pathways in a mix-and-match approach; that is, combining enzymatic activities from different sources in order to establish improved routes in terms of thermodynamics, kinetics, etc. A main effort in the lab is the engineering of alternative one-carbon metabolism, especially with regards to formate assimilation, as we believe formate to be a highly promising feedstock for future cultivation of microbes. We further study and engineer carbon-fixation and related processes, to support higher photosynthetic efficiency.
Prof. Dr. Anke Becker
LOEWE Center for Synthetic Microbiology, Comparative Genomics, Hans-Meerwein-Straße 6, 35032 Marburg , Germany
Anke Becker is Managing Director of the LOEWE Center for Synthetic Microbiology (SYNMIKRO), head of the Comparative Genomics research group, and Professor for Microbiology at the Philipps-Universität Marburg. Her current research centers on bacterial genome architectures and the functional basis and evolution of bacteria-host interactions. Synthetic biology concepts and tools are applied and developed to facilitate megabase scale-DNA assembly and to re-engineer bacterial genomes.
Prof. Dr. Stefan Dübel
Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics, Spielmannstr. 7, 38106 Braunschweig , Germany
The Department of Biotechnology of the Institute for Biochemistry, Biotechnology and Bioinformatics at Braunschweig University covers a broad range of fundamental and applied research topics centered about discovery and molecular engineering of recombinant human antibodies and in vitro evolution. First, antibodies are discovered from our billion-clone libraries by recombinant methods. Using the power of in vitro display technologies, antibodies can be generated with pre-defined biochemical properties and to targets not amenable to animal-based antibody generation. But this is just the start: in analogy to the engineering of machines, antibodies and other protein domains are designed, assembled at the molecular level, tuned and adapted to achieve specific functions in biological systems or technical applications.
This allowed us to contribute to infection biology, cancer research, diagnostic test development, intrabody application, developmental biology, biomarker and drug discovery, synthetic biology, biochemical engineering and process development. A substantial list of successful technology transfers proves the significance of our research for society, by avoiding animal experiments and providing new ways to develop designer medications of the 21st century as well as antibodies tailored to machines.
Prof. Dr. Peter Dürre
Universität Ulm, Institut für Mikrobiologie und Biotechnologie, Albert-Einstein-Allee 11,89081 Ulm , Germany
Syngas (CO/H2 mixtures) and CO2/H2 mixtures, especially from waste gas streams or biomass conversion, can play a major role in biotechnological production processes, combining the advantage of avoiding substrates competing with nutrition (such as starch or sugar) or high crude oil prices (if products are made by chemical synthesis) with climate improvement by reducing greenhouse gases. Metabolic engineering of autotrophic acetogens for production of a number of platform chemicals from gases is performed.
Dr. Tobias J. Erb
Director of the Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology & Center for Synthetic Microbiology, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany
Research in our lab centers on the discovery and design of novel enzymes and metabolic pathways, as well as their transplantation and evolution in natural and artificial cells. One example is the design of synthetic pathways for the capture and conversion of the greenhouse gas CO2 that are more efficient than the photosynthesis of plants and algae. Our ultimate goal is to transform biology from an analytical-descriptive approach into a synthetic-constructive discipline that will allow us to design novel solution for human needs, for instance an increased photosynthesis productivity or the development of synthetic chloroplasts and artificial leavesto harness CO2 as a sustainable carbon source of the future.
Dr. Georg Fritz
Quantitative and Computational Microbiology Group, LOEWE Center for Synthetic Microbiology, 35043 Marburg, Germany
The Quantitative and Computational Microbiology lab focuses on the design and construction of orthogonal gene regulatory circuits, with the ultimate goal of creating robust “software” to drive the expression of heterologous biosynthetic pathways in bacteria. To this end we apply a broad spectrum of experimental, theoretical and computational approaches to harness the potential of Extracytoplasmic Function (ECF) σ factors for orthogonal circuit design. These alternative σ factors split into more than 90 phylogenetically distinct ECF σ groups, which often recognize group-specific target promoter motifs – thereby making them ideal building blocks for developing multiple, orthogonal switches that can be simultaneously used in a bacterial cell. By building increasingly complex ECF “toy circuits”, such as timers and oscillators, we generate a suite of gene expression programs with tailor-made functional characteristics and explore the boundaries of predictive circuit design.
Prof. Dr. Jörg S. Hartig
Department of Chemistry , University of Konstanz, 78457 Konstanz, Germany
The Hartig group is developing artificial genetic switches based on ribozyme catalysis. For this purpose a self-cleaving RNA motif is augmented with an aptamer domain responsible for ligand binding. Engineered, artificial riboswitches have the advantage of allowing conditional gene expression without the need for regulatory proteins. Apart from this topic, the Hartig group is interested in discovering natural post-transcriptional mechanisms of genetic control and in characterizing novel metabolic pathways in bacteria.
Dr.-Ing. Steffen Klamt
Analysis and Redesign of Biological Networks, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
Our group develops and applies (Systems Biology) tools and methods for the model-driven analysis, inference, and targeted modification of cellular networks. One particular focus of our research is computational methods for (systems) metabolic engineering and computer-aided design of microbial cell factories.
As one major outcome of these efforts, we have been developing CellNetAnalyzer, a comprehensive MATLAB package with graphical user interface providing, among others, numerous algorithms for the in silico analysis and computational design of metabolic networks. We apply these methods and tools in realistic applications, e.g., for the model-driven construction of E. coli strains for high-yield itaconic acid production. Furthermore, recent efforts address the use of synthetic regulatory circuits to optimize the productivity of microbial production processes.
Prof. Dr. Dietrich Kohlheyer
Microscale Bioengineering Group, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425 Jülich , Germany
The Microscale Bioengineering Group develops novel microfluidic cultivation systems for microbial research. Disposable devices with specifically designed microenvironments facilitate monolayer cell growth and high environmental control. Automated time-lapse imaging with spatial and temporal resolution delivers new insights into population dynamics at the single cell level.
Prof. Dr.-Ing. Andreas Kremling
TU München, Fachgebiet für Systembiotechnologie, Boltzmannstr. 15 85748, Garching , Germany
Systems Biotechnology at TU Munich focuses on application of methods from systems biology to problems related to biotechnology. Systems biology is a new and interdisciplinary research field that combines mathematical/theoretical approaches with experimental methods. Besides an improvement of the overall biotechnological process the better understanding of the processes inside the cell is a major research topic. Escherichia coli, Pseudomonas putida and Halomonas elongata are chosen as model organisms since they show a high potential with respect to biotechnology.
A methodical focus of the division will be the development of meaningful mathematical models that first describe the behaviour of the cells and secondly support control and design of the system at hand. This requires models with predictive power integrating experimental data from various levels of the cellular organization with process data. For the experimental work, laboratory space is available with good equipment for chemical analysis and cultivation.
Dr. Gerald Lackner
Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute (HKI), Jena, Germany
Our research topics are rare cofactors, natural product biosynthesis and therapeutic microbes. We investigate the physiological and biochemical roles of exotic cofactors in bacteria (e.g. coenzyme F420) to make them available for heterologous pathway expression and biotechnical applications. Furthermore, we develop microbial physicians, i.e. commensal bacteria that sense human pathogens and treat infections by heterologous production of bioactive natural products (e.g. antibiotics) in situ.
Dr. Hannes Link
Dynamic Control of Metabolic Networks, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany
Metabolic networks respond rapidly to internal and external perturbations by changing fluxes, metabolite concentrations and enzyme levels. Our group is interested in the regulation of these responses and their functional relevance in microbes. Therefore, we investigate known metabolic control circuits, map novel regulatory interactions and engineer synthetic metabolic regulation. The ultimate goal is to improve metabolically engineered strains by enabling self-optimization of overproduction pathways for chemicals.
Our research group works on the interface of molecular systems biology and metabolic engineering to understand and manipulate the function of metabolic networks. Our main research areas can be categorized as: i) understanding the function of metabolic control circuits, ii) mapping regulatory interactions between metabolites and proteins, and iii) engineering of metabolic control circuits. An important concept in the group is to enforce changes in the metabolic network by genetic perturbations. For point mutations, deletions and insertions we use Cas9-based genome editing methods, and RNA-based methods for perturbations of gene expression. Inducible repression of metabolic genes, for example, allows us to investigate how cells compensate the resulting metabolic bottlenecks and infer regulators of this process. Time-resolved metabolite and proteome data helps us to distinguish the acute local responses from the more widespread global responses in the metabolic network. Therefore, we use our LC-MS based metabolomics system to measure fluxes and metabolite concentrations, as well as proteomics to probe enzyme expression. Integration of these multi-level data with metabolic models allows us to understand how regulatory mechanisms determine performance and robustness of the metabolic network. The overall goal of our research is to understand fundamental design principles of metabolic control circuits and transfer them to biotechnologically relevant systems. In the long-term we will create synthetic metabolic feedback in order to improve fitness of production strains and thereby maintain high productivity of bioprocesses.
Dr. Jan Marienhagen
Forschungszentrum Jülich GmbH, Institut für Bio- und Geowissenschaften,IBG-1: Biotechnologie,52425 Jülich, , Germany
Aim of the research of the "Synthetic Cell Factories" - group at the Institute of Bio- and Geosciences, IBG-1: Biotechnology (Research Center Jülich, the Helmholtz Association) is the extension of metabolic capabilities of microorganisms for applied purposes by using novel approaches of synthetic biology. Resulting synthetic cell factories should be able to efficiently utilize biomass-derived substrate for synthesizing valuable products: such as (plant) natural products, fine chemicals or pharmaceutical building blocks. In addition, we develop novel tools for the synthetic biologist, which allow for the construction of synthetic operons, the modification of microbial genomes, and high-throughput screening of metabolite-producing bacteria at the single-cell level using biosensors.
Dr. Sylvestre Marillonnet
Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
In my group, we are interested in developing tools for assembly of multigene constructs and optimization of gene expression. We have previously developed a DNA assembly method (Golden gate cloning) and a modular cloning system (MoClo) that allows users to assemble multigene constructs from library of standard biological parts using a series of one-pot assembly reactions. This system also allows the generation of libraries of multigene construct that vary, for example, in the regulatory sequences of the various genes of the constructs, which can be useful for optimization of a biosynthetic pathway. We are also interested in reconstitution biosynthetic pathways for high level production of metabolites of interest in industrial organisms such as plants, yeasts and bacteria.
Prof. Dr. Kerstin Otte
University of Applied Sciences, Biberach, Germany
Therapeutic proteins are highly complex biological drugs which are mainly produced by eukaryotic cell lines. Optimized and tailored mammalian production cell lines are needed for the production of safe and efficient molecules with high quality. In contrast to classical cell engineering strategies, our laboratory focuses on the development of micoRNAs (miRNAs) as versatile tools to genetically optimize mammalian production cells. Our comprehensive miRNA research led to the generation of a ‘miRNA target catalog' providing an avenue for next-generation cell engineering.
Dr. Lennart Randau
Max-Planck-Institut für terrestrische Mikrobiologie, 35043 Marburg, Germany
Lennart Randau is head of the Max Planck Research Group “Prokaryotic Small RNA Biology”. Our research focuses on identifying novel functions of small non-coding RNA molecules in archaea and bacteria. Unique fragmented, circular and double-stranded RNA families have been identified. We characterize minimal type I CRISPR-Cas systems and utilize engineered CRISPR-Cas variants to specifically target and protect RNA molecules in bacterial cells. In addition, we investigate the applicability of archaeal kink-turn elements as synthetic translational regulators. Our group applies and combines biochemical (RNA-seq), microbiological and synthetic biology approaches.
Dr. Tom Robinson
Max Planck Institute of Colloids and Interfaces, Potsdam-Golm, Germany
Understanding the biological cell at its minimal complexity can unravel the role of specific components in existing complex cellular systems. To this end, the “bottom-up” approach of synthetic biology aims to build-up simple biological cell analogues from individual chemical building blocks. The final aim being the creation of a complex but controllable system which reflects natural cellular processes. Our group uses two main tools: the first is biomimetic giant lipid vesicles which we use as artificial cells or proto-cells, and the second is microfluidic technology which provides a platform to create and to handling lipid vesicles. Currently, our research is focused on multi-compartment eukaryotic cell mimicking systems and biomembrane fusion.
Dr.-Ing. Jochen Schmid
Metabolic Engineering & Microbial Polysaccharides group at the Chair of Chemistry of Biogenic Resources, TUM , Germany
The group of Metabolic Engineering & Microbial Polysaccharides headed by Dr.-Ing. Jochen Schmid at the Chair of Chemistry of Biogenic Resources (Prof. Dr. Volker Sieber) of the Technical University of Munich deals with various aspects of synthetic biology. The focus is on the design of synthetic polysaccharide variants. By applying genome editing as well as metabolic engineering approaches new production strains as well as chassis organisms are developed. We use self-designed CRISPR-Cas9 constructs for the development of chassis organisms to produce synthetically in vivo designed polysaccharide variants.
Prof. Dr. Beatrix Süß
Synthetic Genetic Circuits, Technische Universität Darmstadt, Fachbereich Biologie, B2|03 Schnittspahnstraße 10, 64287 Darmstadt
In our group we work on creating novel synthetic RNA aptamers against small molecule targets. We are engineering them to be used as designer riboswitches for the application in a range of living organisms, from Escherichia coli to human cells. These versatile tools are used to create complex genetic circuits to regulate gene expression. Expanding the toolbox of synthetic biology, we aim not only to generate new tools, but also to further the understanding of existing aptamers and riboswitches by studying their RNA biochemistry.
Prof. Dr.-Ing. Ralf Takors
Universität Stuttgart, Institut für Bioverfahrenstechnik, Allmandring 31, 70569 Stuttgart , Germany
The Institute of Biochemical Engineering (IBVT) focuses on the development of novel bioprocesses making intensive use of synthetic and systems biology. Cell-free protein synthesis represents one core activity aiming at the maximization of volumetric productivities based on data-driven models. Industrially attractive strains such as Escherichia coli, Corynebacterium glutamicum and Pseudomonas putida are studied for engineering their fundamental kinetic properties. For instance , key regulatory programs are modulated, genome reduction is performed or novel orthogonal regulatory circuits are implemented – with the goal to create production platforms that are best prepared for large-scale application.
Prof. Dr. Matias Zurbriggen
Synthetische Biologie, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany
Our research perspective is to apply synthetic biology approaches to control and understand eukaryotic signalling processes and regulatory networks in a quantitative and spatiotemporally resolved manner. To this aim we follow an interdisciplinary approach at the interface of engineering and life sciences, focusing on plant and mammalian synthetic signalling networks and metabolic pathways, biological sensors, and chemical and optical switches.