International Genetically Engineered Machine Competition
The iGEM Competition is an annual student team competition in Synthetic Biology. The goal is to solve real-world problems through the design and building of genetically engineered parts and systems. This work is performed over the Summer and presented in a final Jamboree. This page seeks to gather the information from all iGEM teams in Germany in order to broadcast their ideas and achievements to all the SynBio community in this country.
iGEM Team Aachen
Decreasing fresh water availability is not a problem restricted to North-Africa and the Middle East, but also for European countries like Germany or the Netherlands, where water is polluted mainly by industry, causing whole ecosystems to die.
We, the iGEM Team Aachen 2017, consisting of 16 RWTH students, are modifying the genome of Saccharomyces cerevisiae to increase its uptake of different ions and storage inside its vacuole. This will be achieved by upregulation of native (vacuolar-) ion importers and knock-out of ion exporters. Furthermore, we are expressing genes from the plant Arabidopsis thaliana in yeast to improve the yeasts vacuolar ion accumulation and thereby create a microbial dustbin within the cell.
With this newly generated library of genetically engineered yeasts, we hope to offer a new way of treating industrial process water by storing pollutants in our intracellular reservoir, the Salt Vault. This concept can then be used by industries struggling with high pollution in their process water.
iGEM Team Bonn
We are a team of international students, formed by a collaboration of the University of Bonn and the Hochschule Bonn-Rhein-Sieg. This year we are working on an innovative way to prevent caries. We aspire to achieve this goal by expressing surface proteins of the cariogenic pathogen Streptococcus mutans in Escherichia coli. We will perform SELEX on these proteins to obtain aptamers which specifically bind to S. mutans, therefor preventing the formation of biofilm. And thus the formation of Caries.
iGEM Team Potsdam
Since December 2016 we, twelve students, meet regularly to bring our project forward . The iGEM team Potsdam 2017 is the first iGEM team of the University of Potsdam since 2012. It is led by Bryan Nowack and Felix Lohrke (both Bachelor's students in bioscience) and under the supervision of Prof. Bernd Müller-Röber and several Postdocs from the Max Planck Institutes and the University. With metabolic channeling, the speed of the reaction of a metabolic pathway is raised by putting the enzymes close together, thus the diffusion distances for the intermediates are reduced. To use metabolic channeling for the indole-acetic acid pathway, we devised two different approaches. In the first approach, we are utilizing the DNA-binding property of the dCas9-protein to put the enzymes next to each other on a DNA-scaffold. The proteins bind via Aptamers and specific binding proteins to dCas9. This approach is tested in E. coli while the second one will be implemented in yeast. The second approach works with liquid-liquid-phase separation. In this process, membraneless organelles are formed, induced by specific variable domains of Ddx4. We want to investigate, whether fusing the enzymes for the pathway with Ddx4 enables droplet formation and thereby induces metabolic channelling. This could help in making the production of several biotechnological compounds cheaper when used in industrial scale.