GASB

Prof. Dr. Stefan Dübel

Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics
Spielmannstr. 7, 38106 Braunschweig, Germany

 

Title: Exploring the antibody structure space for synthetic biology applications

The development of technical applications using antibodies is frequently hampered
by the limited choice of molecules, when antibodies were generated by classical
animal immunization. Here, recombinant methods based on a complete in vitro
selection by phage display, offer several new opportunities. Phage display allows to
select for Antibodies with pre-defined biochemical properties from the start, as the
biochemical milieu during the in vitro selection can be controlled exactly. Further,
properties of existing antibodies can be changed or improved in various directions to
adapt the molecule to the requirements of a particular technical application, like a
biosensor.
We present examples of successful generation of antibodies binding to extremely
toxic molecules, of antibodies specifically selected to detect very small differences in
the antigen structure, improvement of affinity or stability, and the change of the
kinetic binding properties to enable repeated measurements. Further, we present a
new universal allosteric switch module to regulate the affinity of the antigen binding
site. Such antibodies could in the future, for example, provide target specific claws for
nanomachines.